Paper based microfluidic point of care diagnostic devices pdf

Apr 12, 2016 3d paper based microfluidics have the following advantages over 2d. Paperbased devices are a portable, userfriendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. We will also identify challenges to commercialization. Paperbased pointofcare testing in disease diagnostics. Development of point of care diagnostic assays using paper based microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the point of care has broad implications for the global management of healthcare. Paper based microfluidic systems have emerged as one of the most favorable technologies used in many potential applications such as point of care diagnostics, flexible electronics, energy storage.

Microfluidicbased pointofcare testing for global health tassaneewan laksanasopin. A chemically patterned microfluidic paperbased analytical. Portable, lowcost, and quantitative nucleic acid detection is desirable for pointofcare diagnostics. Newgeneration hospital centers are equipped with centralized diagnostic laboratories containing hightech analytical devices and clinical instruments, which are not feasible or affordable for many lessdeveloped countries. Diseases such as periodontitis and diabetes mellitus can potentially be detected through these devices by the detection of important biomarkers. Paper based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition. Cheap ubiquitous lightweight can be disposed of by incineration, so doesnt produce biohazardous waste. Pads are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Paperbased analytical devices for clinical diagnosis. Pads paper is hydrophilic and porous, thus providing a natural platform for fabricating microfluidic channels. Pads are a new class of pointofcare diagnostic devices that are inexpensive.

To tune fluid penetration in porous substrates such as paper in two and three dimensions, the pore structure, wettability and geometry of the microfluidic devices can be controlled. In fact, they can not only be used for biochemical analysis but also for medical and forensic diagnostics. Paperbased point of care diagnostics posner research group. Pdf a historical perspective on paper microfluidic based. Despite their significant scope, the complexity of fabrication. M bsa concentrations and 0250 mm glucose concentrations was used to investigate the relationship between two assays using the. Speds are inexpensive, lightweight, mechanically flexible, easy to use, and disposable by burning. A microfluidic paper based analytical device pad presents a promising alternative to traditional laboratory tests by allowing for the rapid and lowcost diagnoses of diseases in resource. Threedimensional microfluidic paperbased analytical devices 3d. Integrating microfluidics, optical sensing modality, and smartphones can provide a powerful platform to develop affordable and easily. As a rapidly developing field, paperbased microfluidic devices.

Paper substrates have been widely used to construct point of care lateral flow immunoassay diagnostic devices like the home pregnancy test. Apr 26, 2019 in addition, the basics of paper based microfluidics including a short history of paper based microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described. While many devices focus on one of those four areas of detection, a few devices identifies fungi such as candida. Pointofcare microfluidic devices for pathogen detection. Biomedical engineering a paperbased multiplexed transaminase test for lowcost, pointofcare liver function testing nira r. Currently, the majority of poc diagnostics utilize paper substrates owing to affordability, disposability, and mass production capability. Paper based microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. Integrated paperbased microfluidic devices for pointof. Poc devices have been found to have many advantages like a rapid and precise response, portability, low cost, and non. Since the development of the first commercially available devices in the 1990s, microfluidic technologies have been evolving to benefit an evergreater range of applications. Microfluidicbased pointofcare testing for global health.

The technology builds on the conventional lateral flow test which is capable of detecting many infections agents and chemical contaminants. Paper based microfluidic devices fill a growing niche for portable, cheap, and userfriendly medical diagnostic systems. Paperbased microfluidic pointofcare diagnostic devices. Aug 22, 2017 this work describes the fabrication of self. Moreover, each device can be used to measure more than one analyte. Improving sample distribution homogeneity in three. Numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527. Microfluidic paperbased analytical devices micropads began as a simple idea with an ambitious goal.

Fabrication of miniaturized paperbased microfluidic. Since their introduction in 2007, paperbased microfluidic devices micropads have been explored extensively as platforms for pointofcare diagnostic tests and as tools for basic research and. Paperbased 3d microfluidic device for multiple bioassays. Paperbased systems for pointofcare biosensing sherine. Paper substrates have been widely used to construct pointofcare lateral flow immunoassay diagnostic devices like the home pregnancy test. To listen to a podcast about this feature, please go to. A large part of the excitement behind microfluidics is in its potential for producing practical devices, but surprisingly few labonachip based technologies have been successfully introduced into the market. This platform opens new possible applications for paper based point of care poc diagnostic devices and enables the possibility of these devices to introduce time control functions without the. We define paper broadly as a variety of porous materials, although the emphasis often is on traditional cellulosic forms. Microfluidic systems as point of care diagnostic devices.

Can be produced on a mass scale portable 6 what are microfluidic pointofcare diagnostic devices in the year 2004, the world. Recently, paper has been functionalised as a substrate to construct microfluidic devices for use in rapid diagnostic tests. Apr 26, 2017 a chemically patterned microfluidic paperbased analytical device cpad is developed to create fluidic networks by forming hydrophobic barriers using chemical vapor deposition cvd of. Beattie,2 sidhartha jain,2 farzad noubary,3 vicki l.

Odevelopment of pointofcare diagnostic assays using. Selfpowered integrated microfluidic pointofcare low. Paper based microfluidics rely on the phenomenon of capillary penetration in porous media. This platform opens new possible applications for paperbased pointof care poc diagnostic devices and enables the possibility of these devices to introduce time control functions without the. Microfluidics based devices need a fraction of the sample for data interpretation. Early microfluidicbased pointofcare diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. The goal was to develop lowcost and portable paperbased diagnostic devices to improve healthcare in developing countries. Diagnostics for all, instrumentfree tests based on paper, capillarydriven microfluidics, with a colorimetric readout. Special issue paperbased microfluidic devices for point. The first system, using digital microfluidics, is reusable and allows for precise fluid control. Rise in demand for point of care poc devices is expected to significantly drive the market. Paper based microfluidic point of care diagnostic devices received 6th february 20, accepted 18th march 20 doi.

There was a dramatic rise in the use of paper in pointofcare diagnostics after two reports in 2007 demonstrated that. Digital and paper microfluidic diagnostic devices design document 3 summary the project aims to design two complementary systems for performing biological assays and diagnostic tests. An automated smartphonebased diagnostic assay for point. The future of microfluidic pointofcare diagnostic devices. Making paper diagnostic tests, made while he was working. Pads and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrumentfree fast results. Microfluidic systems as pointofcare diagnostic devices. The integration issues relevant to the creation of paperbased diagnostic devices are analogous to the issues in conventional microfluidicsbased diagnostic device development. There was a dramatic rise in the use of paper in point of care diagnostics after two reports in 2007 demonstrated that. Jun 28, 2010 early microfluidic based point of care diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. Apr 26, 2017 multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home. Pads are a new class of pointofcare diagnostic devices that are inexpensive, easy to use, and designed specifically for use in developing countries. In 2008, the group introduced the first 3d microfluidic paperbased analytical devicesalso referred to as pads.

Paper based microfluidic point of care diagnostic devices. Progress in the development of paperbased diagnostics for. The expanding role of paper in pointofcare diagnostics. Development of pointofcare diagnostic assays using paperbased microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the pointofcare has broad implications for the global management of healthcare. This indicates that the smartphonebased 3d paperbased microfluidic device is a useful platform with great potential for application in pointofcare diagnostics. The main advantage of this is that it is largely a passively. Paperbased microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition. From microfluidic paperbased analytical devices to paper. Pads are expected to have profound implications, especially for in vitro diagnosis. Paperbased microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. Pdf paperbased microfluidic pointofcare diagnostic. Rise in demand for pointofcare poc devices is expected to significantly drive the market.

Jun 23, 2017 paperbased analytical devices for clinical diagnosis. Mar 22, 2017 numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527. Nevertheless, only singleanalyte approaches are typically. Salivary diagnostics on paper microfluidic devices and. Multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home. Whitesides5 in developed nations, monitoring for dr uginduced liver injury through serial. In addition, the basics of paperbased microfluidics including a short history of paperbased microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described. The goal was to develop lowcost and portable paperbased diagnostic devices to. Fabrication of laser printed microfluidic paperbased. Paperbased microfluidic devices fill a growing niche for portable, cheap, and userfriendly medical diagnostic systems.

A accommodates more assays on the same footprint of a device than a 2d device testing of different samples simultaneously within a compact space b moves fluid through the thickness of paper the zdirection and laterally the x,yplane. A paperbased multiplexed transaminase test for lowcost. There is a wide variety of papers that can be employed to build microfluidic devices. However, paper based microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental. But his teams aim is to enable microfluidics to perform a greatly expanded range of tests for everything from infectious diseases to drug. Over the last decade, one particularly promising realworld application to emerge has been point of care poc diagnostics.

Successful device integration can be achieved by a twopart approach composed of a systemlevel view of the whole device and individual module development 84. Microfluidic paperbased analytical devices pads have provided a breakthrough in portable and lowcost point of care diagnostics. Pointofcare poc diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. Paperbased diagnostic microfluidic devices medgadget. Lowe, journallab on a chip, year20, volume 12, pages 221051. Recently, paperbased microfluidics has emerged as a multiplexable pointofcare platform which might transcend the capabilities of existing assays in resourcelimited settings. Development of paper based point of care microfluidic diagnostic devices project number imura0628 monash main supervisor name, email id, phoneadrian neild, adrian. Recently, flexible polymer substrates have been investigated due to. The overall goal of this dissertation is to develop microfluidic diagnostic devices which are practical and reliable for global health.

Pads can provide potentially relevant analysis for biomarker detection in many types of biological fluids, including tear fluids 28, 29, whole blood 30 32, serum 33, urine 34 and saliva. In addition, the basics of paperbased microfluidics including a short history of paperbased microfluidics, a comparison of paperbased and conventional plastic or glassbased microfluidic devices and general requirements for ideal pointofcare testing devices are described. The global microfluidics market size was evaluated at usd. Biological detection biological activities can be recognized by different signal transduction pathways performing on the.